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. Author manuscript; available in PMC: 2021 Jul 9.
Published in final edited form as: Cell. 2020 Jul 2;182(1):177–188.e27. doi: 10.1016/j.cell.2020.05.029

Figure 1. Mapping brain-wide cortico-cortical projections with BRICseq.

Figure 1.

A. In conventional fluorophore-based tracing, a separate brain is needed for each source area. B. In MAPseq, barcoded Sindbis virus is injected into a single source, and RNA barcodes from target areas of interest are extracted and sequenced. MAPseq multiplexes single neuron projections from a single source area. (BC = barcodes). C. In BRICseq, barcoded Sindbis is injected into multiple source areas. BRICseq multiplexes projections from multiple source areas, each at single neuron resolution. D. In the soma-max strategy for soma calling, the cubelet with the highest abundance of a particular barcode is posited to be the cubelet that contains the source of that barcode. E. Distributions of barcode abundance in source cubelets and target cubelets. F. Experimental validation of the soma-max strategy reveals an error rate <0.5%. G. BRICseq pipeline.