Fig. 4.

Effect of PKC activation on protein levels of Gln transporters in whole astrocyte lysates. Levels of LAT2 (A), SNAT2 (B), ASCT2 (C), and SNAT3 (D) in primary cultures of astrocytes treated with PMA (100 nM) for 4, 6, 8, and 24 h in whole cell lysates. Shown are results from typical Western blots and immunostaining images. For each lane, protein levels were adjusted to the levels of β-actin. Results are mean ± SD from three to five independent experiments each performed in triplicate; **P < 0.01 control and ***P < 0.01 control (0 h) versus PMA-exposed cells.