Fig. 8.

PKC stimulation influences astrocytic SNAT3 and Nedd4-2 interaction. Cells were exposure to 100 nM PMA for 1, 2, 6, 8, and 24 h and subjected to immunoprecipitation using an antibody against SNAT3 and were then probed for Nedd4-2. In each experiment, an antibody against control IgG was additionally used for immunoprecipitation as the control for nonspecific binding. Similar results were obtained in three to four independent sets of cultures; *P < 0.05, **P < 0.01, and ***P < 0.001 control (0 h) versus Mn(II)-exposed cells.