FIG 1.

Interaction with STAT4 is conserved across NiV P, V, and W, whereas STAT5 specifically interacts with NiV V. A coimmunoprecipitation (co-IP) assay was performed on HEK293T cells transfected with plasmids encoding the indicated HA-tagged NiV P, V, W, and P N terminus (PNT) protein (2 μg) and FLAG-tagged STAT1 (A), STAT2 (B), STAT3 (C), STAT4 (D), STAT5a and -5b (E), and STAT6 (F) (2 μg). pCAGGS denotes the empty vector control. Co-IP was performed using anti-HA beads. Western blots for NiV and STAT protein expression in whole-cell lysates (WCL) and anti-HA bead elutions (IP: HA) were performed using anti-HA and anti-FLAG antibodies as indicated. Anti-GAPDH blots served as a loading control for the WCL. The immunoblots (IB) are representative of at least three independent experiments.