TABLE 1.

E. coli strains and plasmids used in this study

Strain or plasmid	Genotype and/or characteristicsa	Reference(s) or source
SP15	SP15 with mutation in rpsL (Strr), a derivative of SP15 isolated from spinal fluid of a neonate with meningitis; O18:K1 serotype; colibactin producer	2
Nissle 1917 (EcN)	EcN with mutation in rpsL (Strr), a derivative of the probiotic strain EcN; colibactin producer	35
UTI89	Archetypal uropathogenic E. coli isolated from a patient with cystitis; colibactin producer	64
NC101	Commensal murine adherent-invasive E. coli; colibactin producer; procarcinogenic in different CRC mouse models	15
SP15 ΔclbA	clbA mutant of strain SP15; Strr Kanr	2
SP15 ΔclbN	clbN mutant of strain SP15; Strr Kanr	2
SP15 Δppk	ppk mutant of strain SP15; Strr Chlr	This study
EcN Δppk	ppk mutant of strain EcN; Strr Chlr	This study
UTI89 Δppk	ppk mutant of strain UTI89; Chlr	This study
NC101 Δppk	ppk mutant of strain NC101; Chlr	This study
SP15(pMT3a)	SP15 containing pMT3a; Strr Carbr	This study
P1D10	SP15(pMT3a) with transposon inserted into gene ppk; Strr Carbr Kanr	This study
SP15(pMT3)	SP15 containing pMT3; Strr Kanr	This study
SP15 Δppk-c	SP15Δppk containing pGEN-ppk; Strr Chlr Carbr	This study
EcN clbB::lux	EcN harboring the clbB promoter reporter (luxCDABE) fusion on the chromosome; Kanr	17, 26
EcN clbB::lux Δppk	EcN clbB::lux with gene ppk disrupted; Kanr Chlr	This study
TOP10	Used as host for recombinant plasmids	Thermo Fisher
pCM17	Luciferase-encoding pCM17 vector containing the ompC promoter upstream of the luxCDABE operon	65
pMT3	clbB promoter reporter fusion; Kanr	This study
pMT3a	clbB promoter reporter fusion; Carbr	This study
pGEN-MCS	Vector of gene ppk for complementation of Δppk; Carbr	Gift from Ganwu Li
pGEN-ppk	pGEN-MCS bearing ppk sequence; Carbr	This study

^aStr^r, streptomycin resistance; Kan^r, kanamycin resistance; Chl^r, chloramphenicol resistance; Carb^r, carbenicillin resistance.