TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′)a | Aim |
|---|---|---|
| MT3_PclbB-EcoRI-F | CCGGAATTCCTTTGAACTTATCCATGTTTCC | Cloning of DNA sequence MT3 containing the promoter of clbB |
| MT1_PclbB-BamHI-R | CGCGGATCCCAGAGGTATTATCCATAACCATCAC | |
| MT43_ppk-mut-F | CGCCATAATATCCAGGCAGTGTCCCGTGAATAAAACGGAGTAAAAGTGGTATGTGTAGGCTGGAGCTGCTTCG | Deletion of ppk |
| MT44_ppk-mut-R | GTTATTCAGATTGTTCGAGTGATTTGATGTAGTCGTAAATCGCCAACTGCGCATATGAATATCCTCCTTAGTTC | |
| MT54_pGEN-ppk-HindIII-F | CCGAAGCTTGTACATCGGTGCATTTCGTC | Amplification of ppk plus its putative promoter region |
| MT55_pGEN-ppk-BamHI-R | CGCGGATCCAGGGTTATTCAGATTGTTCGAG |
a
Restriction enzyme sites are underlined, and priming sites for amplifying resistance gene are written in bold.