Table 1.
Dissociation Constants for H2A.Z I and II Acetylated Peptides for 5FW-BPTF Determined by PrOF NMR Binding Experiments
Kd (μM)a |
||
---|---|---|
acetylation pattern | H2A.Z I | H2A.Z II |
unacetylated | NB | NB |
monoacetylated | ||
K4ac | 1300d | 1290 |
K7ac | 1140d | NS |
K11ac | 1300d | 1360 |
K13ac | 1810d | 1730 |
K15ac | NSd | 1700 |
diacetylated | ||
K4ac,K7ac | 990d | 660 |
K4ac,K11ac | 780d | 760 |
K4ac,K13ac | 780 | 790 |
K4ac,K15ac | 630 | 430b |
K7ac,K11ac | 1200d | 780 |
K7ac,K13ac | 520 | 310c |
K4me,K7ac,K13ac | ND | 340 |
K7ac,K15ac | 750 | 370 |
K11ac,K13ac | 1440 | 790 |
K11ac,K15ac | 950 | 880 |
K13ac,K15ac | 830 | 910 |
triacetylated | ||
K4ac,K7ac,K11ac | 1510d | 810 |
K7ac,K13ac,K15ac | 650 | 960 |
NB = nonbinding. NS = nonsaturating. ND = not determined.
Average of two experimental replicates.
Average of three experimental replicates (SD ± 30 μM).
Affinities previously reported by Perell et al.36