FIG 3.

Specificity of individual Entamoeba PCRs in the conventional PCR format. The final sets of primers from four Entamoeba species were evaluated for their species specificity. Each conventional PCR (A, E. histolytica PCR; B, E. dispar PCR; C, E. moshkovskii PCR; D, E. bangladeshi PCR) used a single set of primers (for example, E. histolytica PCR used only an E. histolytica-specific primer set as described for panel A) in the presence of DNAs from four Entamoeba species that originated from 10,000 trophozoites each. Amplified products were run on a 2% agarose gel. In each of the PCRs, we observed species-specific amplification and no cross-amplifications with DNA from other Entamoeba species. The DNA size marker used was a 100-bp ladder (BioLabs, USA).