TABLE 3.

Detection of mixed infections^a

No. of E. histolytica trophozoite DNAs	No. of other Entamoeba trophozoite DNAs	Real-time PCR Cq value
		Duplex-1		Duplex-2		Duplex-3		Tetraplex
		E. histolytica	E. dispar	E. histolytica	E. moshkovskii	E. histolytica	E. bangladeshi	E. histolytica	E. dispar	E. moshkovskii	E. bangladeshi
0.1	10	31.2	28.8	35.5	26.2	33.1	28	No Cq	28.7	26.9	29.6
1	10	27.9	28.4	28.3	26.4	28.4	29.4	28.7	27.8	26.8	29.2
10	10	24.9	27.7	24.9	26	25.3	26.5	25.2	29.3	26.2	27.4
100	10	21.7	32.8	21.7	27.3	22.1	23.7	22.1	34.7	28.9	23.3
1,000	10	18.4	No Cq	18.5	No Cq	28.6	20.7	18.7	No Cq	No Cq	19.9
Blank	Blank	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq
1	100	27.7	25.2	31.2	22.9	28.8	27.3	No Cq	25.1	23.4	27.8
10	100	24.7	24.8	25.1	22.7	25.6	25.9	25.2	24.9	23.3	26.2
100	100	21.7	24	21.6	22.6	22.1	23.6	21.8	24.7	23.2	23.7
1,000	100	18.5	28.6	18.5	23	18.7	20.3	18.7	29.4	24.9	20.5
10,000	100	15.4	No Cq	15.5	No Cq	15.6	17.5	15.8	No Cq	No Cq	17.6
Blank	Blank	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq	No Cq

^aPerformance of E. histolytica real-time PCR in the presence of DNA of other Entamoeba species originating from 10 trophozoites or 100 trophozoites as indicated.