TABLE 3.
Discrepant analysis of 4 samples
| Sample identifier | SAMBAb
|
PHE Colindale assay RdRp Initial (CT) | PHE Cambridge (Wuhan) assay (UTM sample) |
PHE Cambridge (Wuhan) assay (SCoV sample) |
Final result | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Initial result | Repeat result | Initial RdRp (CT) | Repeat RdRp (CT) | E (CT) | Initial RdRp (CT) | Repeat RdRp (CT) | E (CT) | |||
| 1-25 | NEG | NEG | POS (31.18) | POS (28.87) | POS (28.96) | Not tested | Not tested | Not tested | Not tested | POS |
| 2-17 | POS | POS | NEG | NEG | NEG | POS (34.99) | POS (33.98) | NEG | NEG | POS |
| 2-25 | POS | POS | NEG (36.58)a | POS (33.62) | NEG | NEG | NEG | NEG | NEG | POS |
| 2-38 | POS | POS | NEG | NEG | POS (35.09) | POS (34.06) | NEG | NEG | NEG | POS |
a
This specimen was assessed as negative by the Colindale RdRp assay; however, when the assay run’s raw data were reexamined and the normal assay control parameters were relaxed (i.e., threshold and endpoint fluorescence) it was weakly positive (shallow curve), with a threshold cycle (CT) value of 36.58.
b
POS, positive; NEG, negative.