TABLE 2.
Diagnostic accuracy of immunochromatographic tests using RT-PCR as the reference standard
| ICT (company) | Total no. of samples tested | No. of samples with expected positives | No. of samples with expected negatives | Viral target | Sensitivity (% [95% CI]) | Specificity (% [95% CI]) | Positive likelihood ratio (95% CI) | Negative likelihood ratio (95% CI) | Diagnostic odds ratio (95% CI) |
|---|---|---|---|---|---|---|---|---|---|
| Rida Quick Rota/Adeno/Noro Combi (R-Biopharm) | 157 | 43 | 114a | Rotavirus A | 93.0 (80.9–98.5) | 97.4 (92.5–99.5) | 35.3 (11.5–108.3) | 0.072 (0.024–0.214) | 493.3 (95.6–2,544.8) |
| bioNexia Noro/Rota-Adeno (bioMérieux) | 157 | 43 | 114a | Rotavirus A | 93.0 (80.9–98.5) | 100 (96.8–100) | 211.7 (13.3–3,368.9) | 0.080 (0.029–0.218) | 2,649.9 (133.9–52,420.1) |
| Rota+Adeno+Noro combo card (CerTest Biotec) | 157 | 43 | 114a | Rotavirus A | 93.0 (80.9–98.5) | 98.2 (93.8–99.8) | 53.0 (13.4–209.9) | 0.071 (0.024–0.212) | 746.7 (120.3–4,632.6) |
| Immunoquick NoRotAdeno (Biosynex) | 157 | 43 | 114a | Rotavirus A | 93.0 (80.9–98.5) | 100 (96.8–100) | 211.7 (13.3–3,368.9) | 0.080 (0.029–0.218) | 2,649.9 (133.9–52,420.1) |
| Rida Quick Rota/Adeno/Noro Combi (R-Biopharm) | 157 | 46 | 111b | Adenovirus, all species | 54.3 (39.0–69.1) | 100 (96.7–100) | 121.5 (7.6–1,955.2) | 0.459 (0.336–0.627) | 264.5 (15.5–4,511.9) |
| 137 | 26 | 111c | Adenovirus F | 80.8 (60.6–93.4) | 100 (96.7–100) | 178.4 (11.2–2,852.8) | 0.205 (0.097–0.431) | 871.7 (46.5–16,352.3) | |
| 129 | 18 | 111d | Adenovirus non–F | 22. 2 (6.4–47.6) | 100 (96.7–100) | 53.1 (3.0–946.0) | 0.767 (0.597–0.985) | 69.2(3.5–1,352.4) | |
| bioNexia Noro/Rota-Adeno (bioMérieux) | 157 | 46 | 111b | Adenovirus, all species | 54.3 (39.0–69.1) | 100 (96.7–100) | 121.5 (7.6–1,955.2) | 0.459 (0.336–0.627) | 264.5 (15.5–4,511.9) |
| 137 | 26 | 111c | Adenovirus F | 80.8 (60.6–93.4) | 100 (96.7–100) | 178.4 (11.2–2,852.8) | 0.205 (0.097–0.431) | 871.7 (46.5–16,352.3) | |
| 129 | 18 | 111d | Adenovirus non F | 22.2 (6.4–47.6) | 100(96.7–100) | 53.1 (3.0–946.0) | 0.767 (0.597–0.985) | 69.2 (3.5–1,352.4) | |
| Rota+Adeno+Noro combo card (CerTest Biotec) | 157 | 46 | 111b | Adenovirus, all species | 58.7 (43.2–73.0) | 100 (96.7–100) | 131.1 (8.2–2,104.4) | 0.417 (0.297–0.585) | 314.5(18.4–5,371.8) |
| 137 | 26 | 111c | Adenovirus F | 84.6 (65.1–95.6) | 100 (96.7–100) | 186.7 (11.7–2,981.2) | 0.167 (0.072–0.389) | 1,115.0 (58.0–21,445.4) | |
| 129 | 18 | 111d | Adenovirus non-F | 27.8 (9.7–53.5) | 100 (96.7–100) | 64.8 (3.7–1,125.4) | 0.714 (0.536–0.951) | 90.9 (4.8–1,735.2) | |
| Immunoquick NoRotAdeno (Biosynex) | 157 | 46 | 111b | Adenovirus all species | 58.7 (43.2–73.0) | 95.5 (89.8–98.5) | 13.0 (5.3–31.7) | 0.433 (0.306–0.612) | 30.1 (10.3–88.0) |
| 137 | 26 | 111c | Adenovirus F | 84.6 (65.1–95.6) | 95.5 (89.8–98.5 | 18.8 (7.9–44.9) | 0.161 (0.065–0.397) | 116.6(29.0–469.4) | |
| 129 | 18 | 111d | Adenovirus non-F | 27.8 (9.7–53.5) | 95.5 (89.8–98.5) | 6.2 (2.0–19.2) | 0.756 (0.566–1.010) | 8.2 (2.1–32.0) | |
| Rida Quick Rota/Adeno/Noro Combi (R-Biopharm) | 157 | 40 | 117e | Norovirus GI and GII | 87.5 (73.2–95.8) | 99.1 (95.3–100) | 102.4 (14.5–723.2) | 0.126 (0.056–0.286) | 812 (91.8–7,183.6) |
| 137 | 20 | 117f | Norovirus GI | 90.0 (68.3–98.8) | 99.3 (96.0–100) | 123.3 (17.4–873.8) | 0.101 (0.027–0.375) | 1,224.0 (105.6–14,188.1) | |
| 137 | 20 | 117g | Norovirus GII | 85.0 (62.1–96.8) | 99.3 (96.0–100) | 116.4 (16.4–827.9) | 0.151 (0.053–0.429) | 770.7 (75.8–7,831.6) | |
| bioNexia Noro/Rota-Adeno (bioMérieux) | 157 | 40 | 117e | Norovirus GI and GII | 47.5 (31.5–63.9) | 98.3 (94.0–99.8) | 27.8 (6.8–114.1) | 0.534 (0.397–0.718) | 52 (11.3–240.1) |
| 137 | 20 | 117f | Norovirus GI | 30.0 (11.9–54.3) | 98.3 (94.0–99.8) | 17.5 (3.8–80.9) | 0.712 (0.534–0.950) | 24.6 (4.5–134.0) | |
| 137 | 20 | 117g | Norovirus GII | 65.0 (40.8–84.6) | 98.3 (94.0–99.8) | 38.0 (9.3–155.9) | 0.356 (0.196–0.647) | 106.8 (20.0–568.9) | |
| Rota+Adeno+Noro combo card (CerTest Biotec) | 157 | 40 | 117e | Norovirus GI and GII | 45.0 (29.3–61.5) | 98.3 (94.0–99.8) | 26.3 (6.4–108.5) | 0.560 (0.422–0.741) | 47 (10.2–217.3) |
| 137 | 20 | 117f | Norovirus GI | 10.0 (1.2–31.7) | 98.3 (94.0–99.8) | 5.8 (0.9–39.2) | 0.916 (0.790–1.062) | 6.4 (0.8–48.3) | |
| 137 | 20 | 117g | Norovirus GII | 80.0 (56.3–94.3) | 98.3 (94.0–99.8) | 46.8 (11.6–188.1) | 0.203 (0.085–0.489) | 230.0 (38.9–1,358.6) | |
| Immunoquick NoRotAdeno (Biosynex) | 157 | 40 | 117e | Norovirus GI and GII | 42.5 (27.0–59.1) | 96.6 (91.5–99.1) | 12.4 (4.4–34.8) | 0.595 (0.455–0.779) | 20.9 (6.4–67.8) |
| 137 | 20 | 117f | Norovirus GI | 15.0 (3.2–37.9) | 96.6 (91.5–99.1) | 4.4 (1.1–18.1) | 0.880 (0.730–1.061) | 5.0 (1.0–24.2) | |
| 137 | 20 | 117g | Norovirus GII | 70.0 (45.7–88.1) | 96.6 (91.5–99.1) | 20.5 (7.5–55.9) | 0.311 (0.159–0.607) | 65.9 (16.6–262.4) |
Includes 38 adenovirus-positive samples, 37 norovirus-positive samples, and 39 negative samples for rotavirus, adenovirus, and norovirus.
Includes 32 rotavirus-positive samples, 37 norovirus-positive samples, 3 rotavirus plus norovirus-positive samples, and 39 negative samples for rotavirus, adenovirus, and norovirus.
Includes 32 rotavirus-positive samples, 37 norovirus-positive samples, 3 rotavirus plus norovirus-positive samples, and 39 negative samples for rotavirus, adenovirus, and norovirus. Positive samples for non-F adenovirus (n = 18) and adenovirus with undetermined species (n = 2) were excluded from the analysis.
Includes 32 rotavirus-positive samples, 37 norovirus-positive samples, 3 rotavirus plus norovirus-positive samples, and 39 negative samples for rotavirus, adenovirus, and norovirus. Positive samples for adenovirus species F (n = 26) and adenovirus with undetermined species (n = 2) were excluded from the analysis.
Includes 38 adenovirus-positive samples, 32 rotavirus-positive samples, 8 rotavirus plus adenovirus-positive samples, and 39 negative samples for rotavirus, adenovirus, and norovirus.
Includes 38 adenovirus-positive samples, 32 rotavirus-positive samples, 8 rotavirus plus adenovirus-positive samples, and 39 negative samples for rotavirus, adenovirus, and norovirus. Genogroup II norovirus-positive samples (n = 20) were excluded from analysis.
Includes 38 adenovirus-positive samples, 32 rotavirus-positive samples, 8 rotavirus plus adenovirus-positive samples, and 39 negative samples for rotavirus, adenovirus, and norovirus. Genogroup I norovirus-positive samples (n = 20) were excluded from analysis.