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. 2020 Dec 17;59(1):e00583-20. doi: 10.1128/JCM.00583-20

TABLE 1.

MinION sequencing metrics for clinical Mycobacterium tuberculosis NGS performed weekly

Run date (mo/day/yr) No. of samples Failed samples (<40×) Run time (hrs) Starting no. of pores Basecall yield (Gb) Reused cell Times refueled
6/20/19 10 2 48 1,162 16.07 1
6/27/19 22 0 24 1,627 13.61
7/4/19 10 0 24 1,187 13.37
7/10/19 4 0 5 840 2.51 Yes
7/18/19 7 0 8 1,498 4.29
7/26/19 28 0 66 1,689 25.27 1
8/1/19 15 0 23 918 9.05 Yes
8/8/19 13 3 20.5 1,528 11.46
8/15/19 11 0 21.5 1,645 11.3
8/22/19 13 1 24 1,300 9.7 Yes
8/29/19 12 0 24 1,367 9.83 Yes
9/5/18 25 0 48 1,606 19.8 1
9/12/18 14 1 26 1,347 10.26 1
9/19/19 9 0 23.5 967 11.11 Yes
9/26/19 16 0 23 1,183 10.41
10/3/19 9 0 22.5 1,378 12.57
10/11/19 17 2 48 1,072 17.53 Yes
10/17/19 8 0 23.3 1,270 12.31
10/24/19 24 0 48 1,712 22.83
10/31/19 16 0 28 1,469 15.49
11/7/19 8 0 28 987 10.06 Yes 1
11/14/19 5 0 23 774 7.76 Yes
11/21/19 17 0 24 1,229 21.61 1
12/2/19 12 0 26 1,402 14.17
12/5/19 6 0 21 1,246 11.07
12/12/19 19 0 48 1,589 17.27 2
12/19/19 13 0 48 1,494 19.83 1
1/6/20 3 0 24 733 8.57 Yes
1/6/20 23 0 24 1,562 23.67 1
1/9/20 4 0 22 893 8.16 Yes
1/16/20 3 0 24 604 619 Yes
1/23/20 18 0 48 1,599 20.81 2
1/23/20 17 0 72 1,495 24.15 2
Total 431 9