TABLE 1.
MinION sequencing metrics for clinical Mycobacterium tuberculosis NGS performed weekly
| Run date (mo/day/yr) | No. of samples | Failed samples (<40×) | Run time (hrs) | Starting no. of pores | Basecall yield (Gb) | Reused cell | Times refueled |
|---|---|---|---|---|---|---|---|
| 6/20/19 | 10 | 2 | 48 | 1,162 | 16.07 | 1 | |
| 6/27/19 | 22 | 0 | 24 | 1,627 | 13.61 | ||
| 7/4/19 | 10 | 0 | 24 | 1,187 | 13.37 | ||
| 7/10/19 | 4 | 0 | 5 | 840 | 2.51 | Yes | |
| 7/18/19 | 7 | 0 | 8 | 1,498 | 4.29 | ||
| 7/26/19 | 28 | 0 | 66 | 1,689 | 25.27 | 1 | |
| 8/1/19 | 15 | 0 | 23 | 918 | 9.05 | Yes | |
| 8/8/19 | 13 | 3 | 20.5 | 1,528 | 11.46 | ||
| 8/15/19 | 11 | 0 | 21.5 | 1,645 | 11.3 | ||
| 8/22/19 | 13 | 1 | 24 | 1,300 | 9.7 | Yes | |
| 8/29/19 | 12 | 0 | 24 | 1,367 | 9.83 | Yes | |
| 9/5/18 | 25 | 0 | 48 | 1,606 | 19.8 | 1 | |
| 9/12/18 | 14 | 1 | 26 | 1,347 | 10.26 | 1 | |
| 9/19/19 | 9 | 0 | 23.5 | 967 | 11.11 | Yes | |
| 9/26/19 | 16 | 0 | 23 | 1,183 | 10.41 | ||
| 10/3/19 | 9 | 0 | 22.5 | 1,378 | 12.57 | ||
| 10/11/19 | 17 | 2 | 48 | 1,072 | 17.53 | Yes | |
| 10/17/19 | 8 | 0 | 23.3 | 1,270 | 12.31 | ||
| 10/24/19 | 24 | 0 | 48 | 1,712 | 22.83 | ||
| 10/31/19 | 16 | 0 | 28 | 1,469 | 15.49 | ||
| 11/7/19 | 8 | 0 | 28 | 987 | 10.06 | Yes | 1 |
| 11/14/19 | 5 | 0 | 23 | 774 | 7.76 | Yes | |
| 11/21/19 | 17 | 0 | 24 | 1,229 | 21.61 | 1 | |
| 12/2/19 | 12 | 0 | 26 | 1,402 | 14.17 | ||
| 12/5/19 | 6 | 0 | 21 | 1,246 | 11.07 | ||
| 12/12/19 | 19 | 0 | 48 | 1,589 | 17.27 | 2 | |
| 12/19/19 | 13 | 0 | 48 | 1,494 | 19.83 | 1 | |
| 1/6/20 | 3 | 0 | 24 | 733 | 8.57 | Yes | |
| 1/6/20 | 23 | 0 | 24 | 1,562 | 23.67 | 1 | |
| 1/9/20 | 4 | 0 | 22 | 893 | 8.16 | Yes | |
| 1/16/20 | 3 | 0 | 24 | 604 | 619 | Yes | |
| 1/23/20 | 18 | 0 | 48 | 1,599 | 20.81 | 2 | |
| 1/23/20 | 17 | 0 | 72 | 1,495 | 24.15 | 2 | |
| Total | 431 | 9 |