Skin PCR/ESI-MS, blood PCR/ESI-MS, and two-tier antibody test results among 57 participants with early Lyme disease. The denominator used to generate the percentages shown in each box is drawn from the box on the figure directly above it rather than the overall total. Shaded boxes indicate positive results. Visit 1 is at baseline enrollment during acute infection, and visit 3 is 6 months following the end of standard of care treatment for early Lyme disease. Two-tier antibody (Ab) status is characterized as follows: Ab (−), two-tier negative on both acute and convalescent testing; Ab (−/+), two-tier negative on acute testing and positive on convalescent testing; Ab (+), two-tier positive on acute testing.