TABLE 5.
Strains used in this study
| Strain or vector | Host | Marker | Tag | Source |
|---|---|---|---|---|
| H37Rv | Christopher M. Sassetti | |||
| BCG (M. bovis Danish) | ATCC | |||
| E. coli XL-1 Blue | Tetracycline | Stratagene | ||
| pET28a | Kanamycin | Novagen | ||
| pUV15tetO | Hygromycin | Sabine Ehrt | ||
| pMV261 | Kanamycin | Christopher M. Sassetti | ||
| E. coli Rosetta | Novagen | |||
| Rv1720pET28a | Rosetta DE3 | Kanamycin | ||
| Rv1720 D5:A pET28a | Rosetta DE3 | Kanamycin | ||
| Rv1720 D94:A pET28a | Rosetta DE3 | Kanamycin | ||
| ΔRv1720c | H37Rv | Hygromycin | ||
| ΔRv1720c:1720-21pJEB402 | H37Rv | Hygromycin-kanamycin | ||
| BCGΔ1720c | BCG | Kanamycin | ||
| BCG: 1468c pMV261 | BCG | Kanamycin | His | |
| BCG: 1068c pMV261 | BCG | Kanamycin | His | |
| BCG: 1441c pMV261 | BCG | Kanamycin | His | |
| BCG: 2340c pMV261 | BCG | Kanamycin | His | |
| BCG: 3097c pMV261 | BCG | Kanamycin | His | |
| BCGΔ1720c:1468c pMV261 | BCG | Hygromycin-kanamycin | His | |
| BCGΔ1720c:1068c pMV261 | BCG | Hygromycin-kanamycin | His | |
| BCGΔ1720c:1441c pMV261 | BCG | Hygromycin-kanamycin | His | |
| BCGΔ1720c:2340c pMV261 | BCG | Hygromycin-kanamycin | His | |
| BCGΔ1720c:3097c pMV261 | BCG | Hygromycin-kanamycin | His | |
| BCG:Rv1721His-1720 pMV261 | BCG | Hygromycin-kanamycin | His | |
| BCG:Rv1721-1720 flag pMV261 | BCG | Hygromycin-kanamycin | 3×Flag | |
| ΔRv0665 | H37Rv | Hygromycin | ||
| BCG:1720 pUV15 tetO | BCG | Hygromycin | ||
| BCG:1721 pUV15 tetO | BCG | Hygromycin | ||
| BCG:1720-21 pUV15 tetO | BCG | Hygromycin | ||
| BCG:1721His K:A-1720 | BCG | Kanamycin | His | |
| BCGΔ1720:1721 His K:A-1720 | BCG | Kanamycin | His |