Table 1.

(A) Intracellular Moles of 1–4 for the 24 h Incubation in 5 mL of DMEM Media; (B) Results of the Recovery Experiments for the Compounds; (C) Nonspecific Binding of the Compounds with the Cell Culture Plate

A
compda	FBS (%) in 5 mL of DMEM	intracellular uptake (nmol/million cells ± SD)b
1	1	0.069 ± 0.03
	5	0.074 ± 0.03
	10	0.12 ± 0.03
2	1	0.15 ± 0.08
	5	0.18 ± 0.08
	10	0.12 ± 0.09
3	1	6.1 ± 0.1
	5	6.5 ± 0.1
	10	5.7 ± 0.1
4	1	8.7 ± 0.2
	5	6.9 ± 0.1
	10	8.2 ± 0.2

B
		final amount (μmol)b
compdc	initial amount (μmol)	extracellular	intracellular	%recovery ± SDb
1	0.065	0.059 ± 0.000007	0.0012 ± 0.00001	93.0 ± 5.3
2	0.065	0.059 ± 0.00009	0.0004 ± 0.00028	91.2 ± 3.4
3	0.0103	ndd	0.0101 ± 0.000483	97.9 ± 4.7
4	0.115	0.107 ± 0.0119	0.0100 ± 0.00206	101.3 ± 9.8

C
compd	input concentration (μM)	extracted concentration (μM)
1	1	0.035
	10	0.100
	100	0.665
2	2	0.009
	20	0.016
	200	0.719
3	0.2	0.022
	2	0.076
	20	0.128
4	0.2	0.133
	2	0.198
	20	0.222

^aThe input concentrations of 1–4 were 10, 20, 2, and 20 μM, respectively.

^bEach set of data is expressed as mean ± SD (n = 3).

^cCompounds were incubated in 5 mL DMEM media with 10% FBS (1 and 2) or 5% FBS (3 and 4) for 24 h in MDA-MB-231 cells.

^dnd, not determined.