Table 2.
compd | input concentration (μM)a |
solvent extraction efficiency (MeCN/MeOH, v/v = 1/1) |
nonspecific binding with the culture plate (%)b,c |
cell-bound inhibitors at 4 °C (nmol/ million cells)c |
---|---|---|---|---|
3 | 2 | 1.0 | 4.0 ± 0.5 | 1.0 ± 0.01c |
5 | 2 | 1.0 | 8.6 ± 0.8 | 1.0 ± 0.01 |
6 | 2 | 0.7 | 16 ± 2.4 | 0.3 ± 0.02 |
7 | 2 | 0.9 | 0.49 ± 0.052 | 0.0 ± 0.03 |
8 | 2 | 0.8 | 3.9 ± 0.27 | 0.1 ± 0.00 |
All inhibitors (2 μM) were incubated in 5 mL fresh media (DMEM and 5% FBS).
The cell culture plates are CELLSTAR cell culture T-25 flask (Greiner Bio-One, catalogue no. 690160; material: polystyrene).
All experiments were performed in triplicate (n = 3).