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. Author manuscript; available in PMC: 2020 Dec 29.
Published in final edited form as: J Med Chem. 2017 Jan 3;60(1):157–169. doi: 10.1021/acs.jmedchem.6b00923

Table 2.

Relationship between Inhibitor Functional Groups/Substructures and the Binding with the Plasticware, the Extracellular Matrices, and the Nonspecific Binding with the Cell Membrane

graphic file with name nihms-1652902-t0010.jpg
compd input concentration
(μM)a 		solvent extraction efficiency (MeCN/MeOH,
v/v = 1/1)		 nonspecific binding with the culture
plate (%)b,c 		cell-bound inhibitors at 4 °C (nmol/
million cells)c
3 2 1.0 4.0 ± 0.5 1.0 ± 0.01c
5 2 1.0 8.6 ± 0.8 1.0 ± 0.01
6 2 0.7 16 ± 2.4 0.3 ± 0.02
7 2 0.9 0.49 ± 0.052 0.0 ± 0.03
8 2 0.8 3.9 ± 0.27 0.1 ± 0.00
a

All inhibitors (2 μM) were incubated in 5 mL fresh media (DMEM and 5% FBS).

b

The cell culture plates are CELLSTAR cell culture T-25 flask (Greiner Bio-One, catalogue no. 690160; material: polystyrene).

c

All experiments were performed in triplicate (n = 3).