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. Author manuscript; available in PMC: 2020 Dec 29.
Published in final edited form as: ACS Chem Biol. 2017 May 1;12(6):1478–1483. doi: 10.1021/acschembio.7b00125

Figure 2.

Figure 2.

Examples of various types of nucleophilic residues targeted by NHS-ester-alkyne. (A) K230 on Aldob, a catalytic residue within the active site. (B) Y55 on Akr1c6, the catalytic proton donor within the active site. (C) K12 and R105 on Nme1, two positively charged residues involved in binding nucleoside diphosphates. (D) K46 on Gnmt, a surface lysine and known succinylation site. (E) K91 on Pfn1, a surface lysine which forms two salt bridges with aspartate residues at the actin–Pfn1 interface. (F) T31 on Anxa5, a surface threonine near the calcium binding site. PDB file designations are listed in the text. Specific structures are all from human counterparts.