Fig 3. Relative expressions of putatively embryogenesis-related genes in Cryptomeria japonica.

Expressions of six genes encoding (a) leafy cotyledon (LEC), (b, c) wuschel (WUS), (d) germin-like protein (GLP), and (e, f) glutathione-S-transferase (GST) were quantitated. For each gene, expression levels were normalized with that in C4 from the 7th week. Means with different letters are significantly different (Tukey’s multiple comparison test, p < 0.01). Samples with high embryogenic potential (C1 and C5) were indicated in gray. In C6 from the 7th week, the expressions of (c) g34977 and (e) g3323 were not quantified due to the low expressions.