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. Author manuscript; available in PMC: 2021 Dec 3.
Published in final edited form as: Mol Cell. 2020 Nov 17;80(5):779–795.e10. doi: 10.1016/j.molcel.2020.10.041

Figure 2. TAX1BP1 Protein Responds to Proteotoxic Stress and Associates with Insoluble Protein in Neurons.

Figure 2.

(A) Human tissue panel probed for TAX1BP1, OPTN, or NDP52.

(B) Primary rat cortical neurons were treated with the indicated amounts of MG132 for 8 or 18 h, fractionated into RIPA-soluble or -insoluble fractions, and immunoblotted for total Ub.

(C) Fractionated primary rat cortical neurons treated as in (B) were blotted for the indicated proteins.

(D) Primary rat cortical neurons were treated with 1 μM MG132 for18h, after which cells were fixed for imaging and stained with antibodies for TAX1BP1 and Ub; scale bar represents 5 μm. Larger fields of view in Figure S3D.

(E) Neurons derived from human induced pluripotent stem cells (iPSCs) treated with 1 μM MG132 for 18 h, fixed and stained with antibodies targeting TAX1BP1 and Ub; scale bar represents 20 μm. All blots and images are representative of at least three independent experiments. Larger fields of view in Figure S3E.