Fig. 6.

Src kinase inhibition prevents the progression of tubulointerstitial fibrosis in UUO mice. Representative photomicrographs of (A) Masson trichrome- and collagen I-stained kidney sections. The scale bar indicates 50 μm, original magnification x200. (B, C) Quantitative analysis of positively stained areas in the cortex is depicted. (D) Protein expression of fibrotic markers, α-SMA, collagen I, and collagen IV, were analyzed by immunoblotting. (E-G) Expression levels of the indicated proteins were quantified by densitometry and normalized with GAPDH. Values are presented as mean ± SE of 6 mice. *p<0.05 vs. sham mice. †p<0.05 vs. UUO control mice.