Fig. 5.

Chaetocin has ability to induce cell differentiation with decrease of H3K9 methylation in the concentration of Hsp90 inhibition. (A, B) After the incubation of HL60 cells (A) and K562 cells (B) with Chaetocin at different concentrations for 72 h, cells were collected, centrifuged at 1,000 r/min and 4°C for 5 min and washed with PBS for twice. 1×106 cells in each well was added 1ml NBT dye (add 0.5 mg/ml PMA 1 μL) for 1 h at 37°C without light. Cells were collected, and 1 mL 10% SDS (containing 0.04 M HCL) was added for 12 h at 37°C, then the absorbance was detected at the wavelength of 540 nm. Data are presented as the mean ± SD from three independent experiments. (*p<0.05,**p<0.01 vs. DMSO). (C, D) After the incubation of HL60 cells and K562 cells with Chaetocin at different concentrations for 24 h, cells were collected. The protein levels of CEBP/β were analyzed by WB. Results are representative of at least three independent experiments. (E, F) HL-60 and K562 cells were treated with Chaetocin for 24 h. The protein levels of me-H3K9 and SUV39H1 were analyzed by WB. Results are representative of at least three independent experiments.