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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Exp Cell Res. 2020 Nov 18;398(1):112386. doi: 10.1016/j.yexcr.2020.112386

Figure 5. Induction of C-bodies does not require active transcription or translation.

Figure 5.

(A–D) When oocytes are treated with actinomycin to inhibit transcription, the chromosomes contract in length and most of the lateral loops disappear. Staining with an antibody against RPB6 is relatively specific for Pol III. One end of the LBC is indicated by an arrowhead. (E–H) When actinomycin-treated oocytes are cold shocked, the LBCs contract even more (open arrow), but C-bodies are induced as usual (solid arrow). (I–L) When oocytes are treated with cycloheximide to inhibit translation, there is relatively little change in morphology of the chromosomes and C-bodies are induced as in controls (arrows).