Figure 3.

Antioxidants Block AN-Extract Induced IL-6 in IHOK Cells. (a) Three antioxidants (EGCG; 12.5 µM, Glu; 5mM, NAC; 10 µM) were treated with or without AN(30 µg/ml) in serum-free media. After 24 hr, conditioned medium was collected. The supernatants were utilized to detect IL-6. (*p < 0.05 by t test). (b) Cells (1 x 10⁶) were seeded onto 100 mm dishes. Antioxidants (EGCG; 12.5 µM, Glu; 5 mM, NAC; 10 µM) were treated with or without AN (30 µg/ml) in serum-free media. The protein was used to identify EMT-related proteins