Figure 4. Def6 suppresses osteogenesis via endogenous IFNβ-mediated autocrine feedback inhibition.

qPCR analysis of type-I IFN response gene expression (A) and osteoblast marker gene expression (B) during osteoblast cell differentiation in the absence or presence of IFNβ blocking antibody (10 U/ml). Control IgG, 10 U/ml. (C) Alizarin red staining (left panel) and its quantification (right panel) of WT and Def6^-/- calvarial osteoblast differentiation at day 16 in the osteogenic medium in the absence or presence of IFNβ blocking antibody (10 U/ml). Control IgG, 10 U/ml. Data are mean ± SEM. *p<0.05. n.s., not statistically significant.