Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
Def6-/- | PMID:16470246 | RRID:MGI:3628445 | |
| Genetic reagent (M. musculus) |
Ifnar-/- | PMID:8009221 | RRID:MGI:3765898 | |
| Cell line (Homo sapiens) | Platinum-E (Plat-E) | Cell Biolabs | Cat# RV-101 RRID:CVCL_B488 |
Has been authenticated by STR profiling and tested negative for mycoplasma |
| Cell line (M. musculus) | ST2 | DSMZ | Cat# ACC333 RRID:CVCL_2205 |
Has been authenticated by STR profiling and tested negative for mycoplasma |
| Cell line (M. musculus) | MC3T3-E1 | ATCC | Cat# CRL-2594 RRID:CVCL_5437 |
Has been authenticated by STR profiling and tested negative for mycoplasma |
| Antibody | anti-IBP/Def6 (rabbit polyclonal) |
PMID:12923183 | WB (1:1000) IF (1:100) |
|
| Antibody | anti-IFN-α/βRα (mouse monoclonal) |
Santa Cruz Biotechnology | Cat# sc-7391 RRID:AB_2122749 |
WB (1:1000) |
| Antibody | anti-mouse IFN Beta, neutralizing (rabbit polyclonal) |
PBL Assay Science | Cat# 32400–1 RRID:AB_387872 |
10 U/ml |
| Antibody | anti-Stat1 (rabbit polyclonal) |
Santa Cruz Biotechnology | Cat# sc-346 RRID:AB_632435 |
WB (1:1000) |
| Antibody | anti-Stat3 (rabbit monoclonal) |
Cell Signaling Technology | Cat# 12640 RRID:AB_2629499 |
WB (1:1000) |
| Antibody | anti- Phospho-Stat1 (Ser727) (rabbit polyclonal) |
Cell Signaling Technology | Cat# 9177 RRID:AB_2197983 |
WB (1:1000) |
| Antibody | anti- Phospho-Stat1 (Tyr701) (rabbit polyclonal) |
Cell Signaling Technology | Cat# 9171 RRID:AB_331591 |
WB (1:1000) |
| Antibody | anti-Phospho-Stat3 (Tyr705) (rabbit polyclonal) |
Cell Signaling Technology | Cat# 9131 RRID:AB_331586 |
WB (1:1000) |
| Antibody | anti-PKR (mouse monoclonal) |
Santa Cruz Biotechnology | Cat# sc-6282 RRID:AB_628150 |
WB (1:1000) |
| Antibody | anti-p38α (rabbit polyclonal) |
Santa Cruz Biotechnology | Cat# sc-535 RRID:AB_632138 |
WB (1:3000) |
| Antibody | anti-Gapdh (rabbit polyclonal) |
Santa Cruz Biotechnology | Cat# sc-25778 RRID:AB_10167668 |
WB (1:3000) |
| Recombinant DNA reagent |
pMXs-IRES-GFP (plasmid) |
Cell Biolabs | Cat# RTV-013 | |
| Recombinant DNA reagent |
pMXs-mPKR-FLAG-IG (plasmid) |
Addgene | Cat# 101792 RRID:Addgene_101792 |
|
| Sequence-based reagent |
siEif2ak2 LNA | Qiagen | Cat#300600 | 40 nM for knockdown |
| Peptide, recombinant protein |
Recombinant mouse IFNβ | PBL Assay Science | Cat#12400–1 | 10 U/ml |
| Peptide, recombinant protein |
Recombinant Murine TNFα | Peprotech | Cat# 315-01A | 40 ng/ml |
| Peptide, recombinant protein |
Recombinant Murine M-CSF | Peprotech | Cat# 315–02 | 20 ng/ml |
| Commercial assay or kit | VeriKine-HS Mouse IFN Beta Serum ELISA Kit |
PBL Assay Science | Cat#42410–1 | |
| Commercial assay or kit | Mouse IFN Alpha All Subtype ELISA Kit |
PBL Assay Science | Cat#42115–1 | |
| Chemical compound, drug |
FuGENE 6 Transfection Reagent |
Promega | Cat# E2691 | |
| Chemical compound, drug |
TransIT-TKO Transfection Reagent |
Mirus | Cat#MIR2150 | |
| Software, algorithm | HISAT2 | PMID:31375807 | RRID:SCR_015530 | http://daehwankimlab.github.io/hisat2/ |
| Software, algorithm | HTseq | PMID:25260700 | RRID:SCR_005514 | https://htseq.readthedocs.io/en/master/# |
| Software, algorithm | edgeR | PMID:19910308
PMID:22287627 |
RRID:SCR_012802 | https://bioconductor.org/packages/release/bioc/html/edgeR.html |
| Software, algorithm | pheatmap |
https://cran.r-project.org/web/packages/
pheatmap/index.html |
RRID:SCR_016418 | https://cran.r-project.org/web/packages/pheatmap/index.html |
| Software, algorithm | Enrichr | PMID:23586463
PMID:27141961 |
RRID:SCR_001575 | https://maayanlab.cloud/Enrichr/ |
| Software, algorithm | ImageJ (v1.50i) |
PMID:22930834 | RRID:SCR_003070 | https://imagej.nih.gov/ij/index.html |
| Software, algorithm | Graphpad Prism 8 | GraphPad Software | RRID:SCR_002798 | |
| Software, algorithm | Osteomeasure | OsteoMetrics, Inc | ||
| Other | ProLong Gold Antifade Mountant with DAPI |
Thermo Fisher scientific | Cat#P36941 |