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. 2020 Dec 7;26(12):2553–2568. doi: 10.1007/s12298-020-00913-z

Fig. 6.

Fig. 6

In silico prediction of protein partners of SlHyPRP1 and DEA1 and molecular cloning and confirmation of gateway Split-YFP constructs. Predicted protein interaction network of SlHyPRP1 and DEA1 and their associated proteins using STRING software tool (Szklarczyk et al. 2019). A. SlHyPRP1, MST1 and WRKY3 showed interaction by the edges representing protein–protein association through text mining.B. DEA1 showed protein interaction network with Snakin-2 by edges representing text mining and co-expression . C. Schematic representation of split YFP constructs in gateway expression vectors pE-SPYNE and pE-SPYCE. D–F. PCR amplification of Snakin-2, WRKY3 and MST1 with gateway adapter primers from cDNA template of tomato (S. lycopersicum cv. Arka Vikas). G–I. Selection of positive clones of Snakin-2, WRKY3 and MST-1 in gateway expression vectors (pE-SPYNE and pE-SPYCE) through restriction analysis. J. Sanger sequence alignment results of positive clones of Snakin-2, K.WRKY3, and L.MST-1 in pDONR vector (pDONR221)