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. 2020 Dec 16;11:602782. doi: 10.3389/fpls.2020.602782

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Copyright © 2020 Jiang, Tang, Shi, Ke, Wang, Che, Luan and Hou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SL1 is expressed in green tissues and localized in the chloroplast stroma. (A–H) GUS staining of SL1_Pro::GUS transgenic plants, showing that SL1 is expressed in the (A) germinating seed, (B) 3-day-old seedling, (C) 7-day-old seedling, (D) rosette leaf, (E) bud, (F) flower, (G) cauline leaf, and (H) silique. Three independent biological repetitions were performed, and three individual plants were used for each repeat. (I) Relative expression of SL1 in different tissues, including the root, cauline leaf, flower, rosette leaf, stem, and silique, at the heading stage. Experiments were replicated three times, with similar results obtained. (J) Confocal microscopic images of the SL1-YFP fusion protein expressed transiently in tobacco epidermal leaf cells. Left to right: yellow fluorescent protein (YFP), chlorophyll, merged image. Bars = 10 μm. (K) SL1 is located in the chloroplast stroma. Chloroplasts were purified from SL1-Flag transgenic plants, and the immunoblot analysis was conducted using antibodies against Flag, ClpC (a stroma protein), D2 (a thylakoid membrane protein), and Cyp38 (a thylakoid lumen protein). C, intact chloroplast; S, stroma; T, thylakoid membrane; and L, thylakoid lumen.