FIGURE 3.

Safranal downregulates the post-transcription of c-MYC and the transcription of Skp2 during cell cycle re-entry in Pca cells. Quiescent LNCaP (A,C) and PC-3 (B,D) cells were stimulated to re-enter the cell cycle in the presence or absence of safranal (GI₅₀ or GI₉₀) at the indicated intervals. The protein and mRNA levels of c-MYC, Skp2, p21, and p27 were then analyzed using immunoblotting (A,B) and RT-PCR (C,D) at each interval. β-actin served as a loading control. Cont, control cells in a proliferative state. Results are expressed as mean ± SD of triplicate assays. ^###P < 0.001 vs. non-quiescent control or *P < 0.05, ***P < 0.001 vs. dimethyl sulfoxide vehicle control.