Acute functional tolerance (AFT) to ethanol-induced rotarod ataxia is reduced in male Tlr3
−/− mice and increased in male Myd88
−/− mice compared with their corresponding wild-type littermates. (A) Time to regain the ability to remain on the rotarod for 60 seconds after the first (1.75 g/kg) and second (2 g/kg) ethanol injections in Tlr3
+/+ and Tlr3
−/− male mice (n = 7-9 per genotype). (B) Blood ethanol concentrations (BECs) measured at the time of regaining motor function after the first and second ethanol injections. (C) AFT measured as the difference in BEC after the two ethanol injections (BEC2 - BEC1). (D) Time to regain the ability to remain on the rotarod for 60 seconds after the first (1.75 g/kg) and second (2 g/kg) ethanol injections in Myd88
+/+ and Myd88
−/− male mice (n = 7-9 per genotype). (E) BECs measured at the time of regaining motor function after the first and second ethanol injections. (F) AFT measured as BEC2 - BEC1. *p < 0.05, **p < 0.01, and ****p < 0.0001 compared with corresponding wild-type mice by Bonferroni post-hoc (A,B,D,E) or two-tailed t-tests (C,F).