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. Author manuscript; available in PMC: 2022 Jun 1.

Published in final edited form as: J Neuroimmune Pharmacol. 2020 Jun 30;16(2):403–424. doi: 10.1007/s11481-020-09933-8

Figure 3. — DCs from three donors were isolated as in Figure 1. DCs were cultured with and without LPS for 24h followed by 3h of 20μM Apigenin treatment. DCs were then co-cultured with autologous PBLs for 6 days with Brefeldin A and Monensin treatment in the last 16h of the co-culture. T-cell phenotype was elucidated by staining for various cell surface markers and transcription factors and the cells were analyzed by flow cytometry. A) FACS plots of T-cell subset-specific expression of transcription factors or cytokines. B) Quantitative representation of FACS plots shown in A (N=3). Statistical significance was determined by Student’s t-test (*p<0.05, **p<0.01, ***p<0.001).

Figure 3. — DCs from three donors were isolated as in Figure 1. DCs were cultured with and without LPS for 24h followed by 3h of 20μM Apigenin treatment. DCs were then co-cultured with autologous PBLs for 6 days with Brefeldin A and Monensin treatment in the last 16h of the co-culture. T-cell phenotype was elucidated by staining for various cell surface markers and transcription factors and the cells were analyzed by flow cytometry. A) FACS plots of T-cell subset-specific expression of transcription factors or cytokines. B) Quantitative representation of FACS plots shown in A (N=3). Statistical significance was determined by Student’s t-test (*p<0.05, **p<0.01, ***p<0.001).