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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2020 Jun 30;16(2):403–424. doi: 10.1007/s11481-020-09933-8

Figure 4. Antigen dependent assay with splenocytes from EAE mice.

Figure 4.

Single cell suspension was prepared from mouse spleen of EAE and naïve mice (n=3). Cells were stimulated with and without MOG35–55 peptide for 3 days in the presence of absence of 20μM Apigenin. Gene expression was compared to β-Actin and normalized to cells that were not treated with MOG35–55 peptide. T-bet, IFN-γ, IL-4, IL-17, and TGF-β mRNA expression for three EAE and three naïve mice were detected by qPCR. Statistical significance was determined by 2-way ANOVA using Sidak’s test for multiple comparisons (*p<0.05, ***p<0.001).