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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2020 Jun 30;16(2):403–424. doi: 10.1007/s11481-020-09933-8

Figure 7. RelB silencing may potentiate Apigenin effects on phenotype of mature DCs.

Figure 7.

DCs from three donors were isolated as in Figure 1. DCs were cultured with and without LPS for 24h followed by treatment with 20μM Apigenin. 25nM siRNA directed against RelB was transfected in to DCs. sip65 was used as positive control. A) Live single cell populations were gated for Lin-1−CD11c+HLA-DR+ DCs. B) Changes in DC phenotype were determined by flow cytometry. Statistical significance was determined by 2-way ANOVA using Sidak’s test for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).