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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2020 Jun 30;16(2):403–424. doi: 10.1007/s11481-020-09933-8

Figure 8. RelB silencing boosts DC-mediated regulation of T-cell differentiation in presence of Apigenin.

Figure 8.

DCs from 3 donors were isolated as before and LPS-matured followed by treatment with 20μM Apigenin. 25nM siRelB was transfected in to DCs. sip65 at 25nM was used as positive control. 36h post transfection DCs were co-cultured with autologous PBLs for 6d. Cytokine secretion was blocked in the final 16h of culture by the addition of Brefeldin A and Monensin. Flow cytometry analysis of cell surface markers and intracellular transcription factors and cytokines was then performed. A) Live single cell populations were gated for CD3+CD4+ T cells. B) Enumeration of the various T-cell subsets upon both RelB knockdown and Apigenin treatment. Statistical significance was determined by 2-way ANOVA using Sidak’s test for multiple comparisons (*p<0.05, **p<0.01).