Figure 5.

cGAS protects mice against P. aeruginosa in pulmonary infection

(A) C57BL/6J WT and cGAS^−/− mice were challenged with 0.5 × 10⁷ CFU PAO1. Survival of mice was monitored up to 7 days. Kaplan-Meier survival curves were obtained using GraphPad. p = 0.0242.

(B) C57BL/6J WT and STING^−/− mice were challenged with 0.5 × 10⁷ CFU PAO1. Survival of mice was monitored up to 8 days. Kaplan-Meier survival curves were obtained using GraphPad. p = 0.0273.

(C) Pathology of WT, cGAS^−/−, STING^−/−, and IFNAR^−/− mice in response to PAO1 infection. H&E staining of lung sections from mice. Original magnification ×200.

(D) ELISA analyzes cytokine levels of IFN-α in WT and cGAS^−/− mice lung.

(E) ELISA analyzes cytokine levels of TNF-α, IL-1β, and IL-6 in in WT and cGAS^−/− mice lung BALF.

(F) ELISA analyzes cytokine levels of IFN-β in WT and cGAS^−/− mice BALF, lung, and blood.

(G) ELISA analyzes cytokine levels of TNF-α and IL-1β in WT and STING^−/− mice lung.

ELISA and qPCR data (mean ± SEM) are representative of three independent experiments (∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001 one-way ANOVA with Tukey post hoc test).