Fig. 6.

Genomic GLO1 deletion antagonizes EMT-related gene expression with suppression of metastasis in a murine melanoma. (a) NanoString nCounter™ pathway score analysis of ‘EMT to metastasis’, ‘metastasis response’, and ‘metastasis suppression’ (box plot depiction). (b) NanoString nCounter™ single gene depiction of EMT- and metastasis-related gene expression. (c) Invasion through Matrigel-coated Boyden chambers (GLO1_WT; GLO1_KO [B40]); bar graph (left panel) depicts numerical analysis. Left panel also displays MMP9 protein levels in conditioned medium (determined by ELISA analysis); right panel: representative images obtained after crystal violet staining of inserts. (d) Melanoma cells (A375 GLO1_WT; GLO1_KO [B40]) were tail vein injected (five SCID mice per group) followed by analysis of lung metastasis 21 d later (top panel: experimental scheme). Representative lung specimens are depicted (right panels). Bar graph summarizes numerical analysis of metastases per lung (left panel). For bar graphs comparing two groups only, statistical significance was calculated employing the Student's two-tailed t-test (*p < 0.05). Nonparametric data analysis of murine experimentation was performed using the Mann–Whitney test (*p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)