Fig. 7.

Genomic GLO1 deletion accelerates tumor growth in a SCID mouse xenograft model of human A375 malignant melanoma. (a) A375 melanoma cells (three groups: GLO1_WT; GLO1_KO [B40]; GLO1_KO [C2]) were injected subcutaneously (ten mice per group) followed by assessment of tumor growth over a 28 d period; top panel: injection scheme; bottom panel: tumor burden as a function of genotype and time. (b–d) At the end of the experiment, tumors were processed for gene expression analysis by RT-qPCR and immunohistochemical staining. (b) Representative tumor images (dorsal, right flank, s. c.; top panels) with Ki67 immunohistochemical analysis of tumor specimens (bottom panels; 20x magnification) as summarized by bar graph depiction (right panel). (c) Immunohistochemical analysis of tumor specimens (GLO1_WT; GLO1_KO [B40]; 20x magnification); quantitative analysis as summarized by bar graph depiction (right panels). (d) RT-qPCR assessment of gene expression as a function of tumor GLO1 genotype (GLO1_WT; GLO1_KO clones [B40 and C2]). For all bar graph depictions, quantitative data analysis employed ANOVA with Tukey's post hoc test; means without a common letter differ from each other (p < 0.05). For bar graphs comparing two groups only, statistical significance was calculated employing the Student's two-tailed t-test (*p < 0.05). Nonparametric data analysis of murine experimentation was performed using the Mann–Whitney test (*p < 0.05).