Fig. 2.

Stable knock-out or transient knock-down of NQO1 did not increase the levels of acetyl α-tubulin in 16HBE or 3T3-L1 cells. (A) Acetyl α-tubulin levels were measured in parental 16HBE cells and two stable NQO1-null clones (D7 and D19) generated using CRISPR/cas9 directed gene silencing. β-actin was included as a loading control. (B) Acetyl α-tubulin levels were measured in (B) 16HBE cells and (C) 3T3-L1 fibroblasts after treatment with non-targeting control siRNA or siRNA targeted to NQO1 for 48 h.