Figure 1.
Monitor of H9N2 virus shedding, HI antibody level, and T lymphocyte percentage after infection. Four chickens of infected and control groups were randomly selected for sampling to detect the viral load of cloacal swabs and oropharyngeal swabs (A), H9N2 HI antibody level (B), the percentage of CD3+CD8+ T cell (D), the percentage of CD3+CD4+T cell (E), the percentage of CD3+CD4+CD8+ T cell (F), and the ratio of CD3+CD4+ / CD3+CD8+ (C). Each sample collected 1 × 105 cells for flow cytometric analysis. H9N2 virus shedding and H9N2 HI antibody in the control group were all negative at all time points (data not shown). The one-way or two-way ANOVA was used for statistical comparison. ns P > 0.05, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. Abbreviations: DPI, days postinfection; HI, hemagglutination inhibition.