Differential serum proteins between the brooding and laying stages. (A)Volcano plot showing the DSP detected in the serum between the laying stage and brooding stage. The X-axis refers to the log2 fold change of the proteins in the brooding stage compared to the laying stage, while the Y-axis shows the −log10 (adjusted P-value) of that comparison. DSP (fold change ≥ 1.5, P-value < 0.001, FDR < 0.05) are in red and the other in gray. (B) Function analysis of downregulated proteins in the broody stage. (C) Function analysis of upregulated proteins in the broody stage. Proteins in column and function terms in row. Abbreviations: DSP, differential serum proteins; FDR, false discovery rate.