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. 2020 Sep 25;100(1):19–25. doi: 10.1016/j.psj.2020.09.034

Figure 2.

Figure 2

IFA on CEF and DF-1 cell cultures infected with MDV, REV, and ALV. Plaques from the isolate GX18NNM5 were evident at 136 h (F2) after inoculation; specific staining of the viral plaques with MDV-1 gB-specific monoclonal antibody BA4 was observed (A, 100 ×), whereas no specific fluorescence was observed in the noninfected cells (D, 100 ×). Positive fluorescence was observed in the GX18NNR1-infected cells by the IFA with REV-specific monoclonal antibody 11B118 (B, 200 ×); no specific fluorescence was observed in the noninfected cells (E, 100 ×). No specific fluorescence was observed in the DF1 cells of both the inoculated cells (C, 200 ×) and the negative control (F, 100 ×) when the IFA was run by using the monoclonal antibody IE5 specific to ALV-p27. Abbreviations: ALV, avian leukosis virus; CEF, chicken embryo fibroblast; IFA, immunofluorescent assay; MDV, Marek's disease virus; REV, reticuloendotheliosis virus.