OTU analysis and alpha diversity index analysis among groups. (A) Venn diagram based on OTU and Box plot of (B) Chao1 index, (C) Shannon index, and (D) Simpson index intergroup differences among groups. Means without a common letter significantly differ (P < 0.05). Control, broilers were fed a basal diet and injected with saline; CPM, CPM-injected broilers were fed a basal diet; IAPS, CPM-injected broilers were fed a basal diet containing 900 mg/kg IAPS. Abbreviations: CPM, cyclophosphamide; IAPS, 60Co γ-ray irradiated Astragalus polysaccharides.