Figure 1.
The effects of a high-fat diet and osteocalcin on the pathological changes of the liver in aged hens. (A–C) Examples of liver light microscopical structures were examined with HE staining. (D–F) The hepatica fibrosis change was observed by Masson's trichromatic staining. (G) Percentage of the fibrosis areas. Bar = 50 μm. →: fibrosis. a, bMean ± SEM with different small letter differ significantly (P < 0.05, n = 10). Abbreviations: HE, hematoxylin and eosin; HFD + OCN, high-fat diet + osteocalcin; HFD + V, high-fat diet + vehicle; ND + V, normal diet + vehicle.