Table 2.
Real-time PCR primers and amplified PCR product size.
| Gene | GenBank ID | PCR primers sequence (5′-3′) | PCR products (bp) |
|---|---|---|---|
| IL-1 | NM_204524.1 | F: GGTCAACATCGCCACCTACA R: CATACGAGATGGAAACCAGCAA |
86 |
| IL-6 | NM_204628.1 | F: AAATCCCTCCTCGCCAATCT R: CCCTCACGCTCTTCTCCATAAA |
106 |
| TNF-α | NM_204267.1 | F: GGACAGCCTATGCCAACAAG R: ACACGACAGCCAAGTCAACG |
168 |
| GAPDH | NM_204305.1 | F: TTGACGTGCAGCAGGAACAC R: ATGGCCACCACTTGGACTTT |
124 |
Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1, interleukin-1; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha.