Figure S2. Plasma membrane targeting of HTR6 mutants.

The indicated HTR6 constructs, all containing C-terminal EGFP, were expressed in IMCD3 cells, which were analyzed by immunofluorescence with antibodies against EGFP (green), acetylated tubulin (AcTub, left) or ARL13B (right) (red) and gamma-tubulin (γTub, magenta). Cells were also stained with DAPI (DNA). All HTR6 constructs clearly label the plasma membrane. Scale bar, 5 μm.