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(A) Lysates from HEK293T cells expressing the proteins indicated on top were immunoprecipitated with anti-Flag antibodies and analyzed by Western blot with anti-HTR6 and anti-Flag antibodies, as indicated. (A, B) Experiment as in (A) except that effect of CT-mut3 was tested in myc-HTR6 rather than myc-Chimera J, as indicated. Molecular weight markers are shown on right of all panels.
