Table 1.
Summary of key results.
| Sufficiency for cilia targeting (Figs 3, 6, and 7) | Residues required for cilia targeting (Figs 4, 5, and 7) | TULP3 | RABL2 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| IC3/CT association (Fig 9) | CTS effect on associationa (Fig 10) | Requirement for CTS function (Fig 11) | IC3/CT association (Fig 12A–C) | CTS effect on associationb (Fig 12A–C) | Requirement for CTS function (Fig 12G–J) | ||||
| HTR6 | IC3 | + | RKQ...V (aa 216–222) | − | n.a. | Strong | ++ | Positive | Strong |
| CT | + | LPG (aa 400–402) | + | Negative | Strong | ++ | −/+ | Weak | |
| SSTR3 | IC3 | + | AP[AS]CQ (aa 243–255) RRRRSERR (aa 256–263) | − | n.a. | ? | ? | ? | ? |
| CT | + | FK (aa 329–330) LL.P (aa 337–340) | +++ | ? | ? | ? | ? | ? | |
a
n.a., not applicable.
b
Effects of mutating CTS2 in HTR6-CT were context-dependent and not obvious (see Fig 12A–C for more details).