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. 2020 Dec 29;4(3):e202000825. doi: 10.26508/lsa.202000825

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© 2020 Alvelos et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S6. — (A, B) Alternative splicing events in the diabetes susceptibility genes GIPR and HMGB that were detected by RNA-seq following SRSF6 KD in EndoC-βH1 cells. Semiquantitative RT-PCR analysis for GIPR did not show a statistically significant difference between the two conditions. RT-PCR amplification for HMGB only yielded one PCR amplicon. Representative digital gel images (top) and quantification (GIPR) are shown. (C, D) The alternative splicing events in the diabetes susceptibility genes CDK2, CENPO, and RBM6 show no significant differences between control conditions (siCTL) and GLIS3 KD (siGLIS3). The event in STARD10 shows a statistically significant change in the opposite direction as observed after SRSF6 KD (Fig 4E). The presence of only one band for the BCAR1 precludes detection of alternative splicing changes. Visualization as in (A).

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