Fig. 5.

Oxysterol-stimulated astrocytes compromise neuronal health. Primary neurons (13 DIV) were treated with the Late AD oxysterol mixture (10 μM) for 24 h or they were co-cultured for 24 h with astrocytes grown on cell culture inserts that had been previously treated with the same oxysterol mixture for 12 h. (A) Graphical representation of co-culture experiments. (B) The protein levels of the postsynaptic density protein 95 (PSD95), synapsin, and cleaved caspase-3 were determined by Western blotting. Data were normalized to the corresponding β-actin levels. Data are expressed as mean values ± SD of three different experiments as percentage change from respective control (Student's t-test). **P < 0.01 and *P < 0.05 vs control.