Figure 1.

Ablation of Cancer Cell-Intrinsic COX Alters the Intratumoral Accumulation of Select Innate Immune Cell Subsets

(A) Kaplan-Meier plots showing the fraction of tumor-bearing wild-type (n = 10), Tmem173^−/− (n = 5), Cgas^−/− (n = 5), Trif^−/− (n = 7), Mavs^−/− (n = 6), and Myd88^−/− (n = 6) mice injected with Ptgs^−/− or Ptgs^+/+ melanoma tumor cells in wild-type (n = 10) hosts.

(B–D) Immune cell infiltrate analysis of Ptgs^+/+, Ptgs^−/−, and Ptgs^−/−+COX-2 tumors 4 days post-implantation. (B) Two-dimensional distributed stochastic neighbor embedding (t-SNE) projections of CD45⁺ cells for each group (n = 6 concatenated samples per group). The frequency and the number of intratumoral neutrophils (C) and NK cells (D) are shown.

(E) Weight of Ptgs^+/+ and Ptgs^−/− tumors 4 days post-implantation in wild-type, NK cell-depleted, or Rag1^−/− mice.

(F) Ptgs^+/+, Ptgs^−/− (+/− synthetic PGE2), and Ptgs^−/−+COX-2 melanoma cells tested for susceptibility to NK cell-mediated killing. E:T refers to ratio of effector:target cells.

(G) Percentage of NK cells contacting either 1 or more (2–5) Ptgs^+/+, Ptgs^−/−, or Ptgs^−/−+COX2 targets.

(H) Violin plots representing the number of interactions and the cumulative contact time of NK cells with Ptgs^+/+, Ptgs^−/−, and Ptgs^−/−+COX-2 targets. Data are expressed as mean ± SEM, one-way ANOVA (C–F and H) or Fisher’s exact test. (G).